منابع مشابه
Light-driven Enzymatic Decarboxylation
Oxidoreductases belong to the most-applied industrial enzymes. Nevertheless, they need external electrons whose supply is often costly and challenging. Recycling of the electron donors NADH or NADPH requires the use of additional enzymes and sacrificial substrates. Interestingly, several oxidoreductases accept hydrogen peroxide as electron donor. While being inexpensive, this reagent often redu...
متن کاملEnzymatic decarboxylation of oxalic acid.
Large quantities of oxalic acid accumulate in the cell sap of various plants and as a major product of carbohydrate metabolism by many molds. A recent investigation in our laboratory revealed that oxalic acid was produced from oxalacetic acid by an enzyme system which was isolated from the mycelia of Aspergillus niger (1). It was also demonstrated that oxalic acid was converted to formic acid a...
متن کاملEnzymatic Decarboxylation of Oxalic Acid* by William
Although it has been recognized that oxalic acid is decomposed by a variety of microorganisms (2-6), relatively little is known of the details of oxalic acid metabolism. The present study represents an attempt to establish the mechanism of oxalate degradation at the enzyme level by the use of a bacterium isolated from soil. Partially purified enzyme preparations obtained from the organism catal...
متن کاملDetermination of aspartic and glutamic acids by enzymatic decarboxylation.
Suspensions of Clostridium welchii (strain SR 12) quantitatively decarboxylate the L isomers of aspartic (1) and glutamic acids (2) to ar-alanine and y-aminobutyric acid, respectively. Although a quantitative procedure for the determination of glutamic acid based upon decarboxylation with this organism has been described (3), it has been found that aspartic acid, especially in the presence of c...
متن کاملThe non-enzymatic decarboxylation of diketosuccinate and oxaloglycolate (dihydroxyfumarate).
Malic dehydrogenase has been shown to catalyze the reduction of diketosuccinate by DPNH (4). Furthermore, both oxaloglycolate and diketosuccinate undergo spontaneous decarboxylation in aqueous solution. These nonenzymatic reactions complicate the analysis of the metabolic path of degradation of tartrate. As a prerequisite for the use of labeled compounds to study the biochemical synthesis and d...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2016
ISSN: 1940-087X
DOI: 10.3791/53439